From Two-dimensional Electrophoresis (2-DE) to Clinical Proteomics Research

Patcharee Isarankura-Na-Ayudhya

Abstract


In the Post-Genomic Era, much attention has been focused on the use of protein as potential targets not only for understanding the underlying mechanisms of disease formation and progression but also for evaluating its applicability as novel biomarkers and drug/vaccine targets. This review article summarizes a general concept of proteomics and its significance in clinical research. Proteomics is widely accepted as the large-scale comprehensive study of protein structures and functions in a desired cell, tissue or organism. Proteomics can be divided into many types e.g. expression proteomics, functional proteomics and structure proteomics. Two-dimensional electrophoresis (2-DE) is a major technique used for protein separation in proteomics studies. The principle is based on separation of proteins by their isoelectric points (pI) in the first dimension and their molecular weights in the second dimension under electrical fields. Detail explanations together with the critical success factors of performing 2-DE (at the step-by-step) are also highlighted. Finally, applications of proteomics in clinical research and their future perspectives are discussed.

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