Proteomics Study of Palmitic Acid-induced Insulin Resistance in Adipocyte Secretome

Kanyanath Pium-ngam, Pilaiwan Siripurkpong, Sudawadee Kongkhum, Sittiruk Roytrakul

Abstract


Insulin resistance is a major cause of type 2 diabetes. Early detection of insulin resistance will be useful to help screening and prevention of type 2 diabetes. The purpose of this study was to explore the differentially  expressed secretome of insulin resistance induced 3T3-L1 adipocytes, by using 1 mM palmitic acid/1% BSA for 2 h, compared to control in order to find biomarker(s) for diagnosis of insulin resistance. The secreted proteins were analyzed by GeLC-MS/MS and the raw MS/MS data were further quantified by DecyderMS and identified by Mascot. Quantitative protein expression was obtained from 245 proteins (p < 0.05) 120 of which were found only in the induced group. These proteins are known to be involved in cellular processes, cellular component organization, regulation, development processes, cell proliferation, interaction with cells and organisms, metabolic processes and immune system proceses but most are associated with metabolic processes, cellular processes and regulation. These proteins are interesting for their potential as  biomarker(s) of insulin resistance.


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