A Preliminary Study of The Real Time PCR Method for Streptococcus suis Detection in Clinical Blood Sample

Jate Wantang, Wittaya Wangsomboonsiri, Warangkana Oncoung

Abstract


Streptococcus suis (S. suis) infection is a zoonosis causing septicemia, meningitis, endocarditis and Streptococcal toxic shock syndrome.  Most patients are infected by eating raw contaminated pork or pork-derived products. The severe cases of S. suis infection may develop rapid disease progression with short incubation time and cause of dead within 24-48 hours. To overcome the critical infection, a better and rapid diagnostic method with high accuracy is needed. In 2012, we optimized a real time PCR assay for detection of S. suis in human blood samples. This study aimed as a preliminary study to compare the recently developed method to the standard bacterial culture method using sixty-seven clinical blood samples from septicemia patients in Sawanpracharak Hospital, Nakhonsawan Province, between August 2012 and October 2013. The results showed that of the total 67 samples, 8 (11.9%) were positive by real-time PCR, while 5 (7.5%) were positive by culture method. The sensitivity, specificity, positive and negative predictive values as well as accuracy of both methods were analyzed. The values for real-time PCR were 80, 100, 100, 96.61 and 97.01% respectively, while those of the bacterial culture method were 50, 100, 100, 91.94 and 92.54% respectively. The results indicated that the real-time PCR method is 30% more sensitive than the bacterial culture method for detection of S. suis in human blood samples.

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