A Hematological Control Product for Automated
In-house hematological control products for automated blood cell analyzer were prepared from citrate-phosphate-dextrose (CPD) fresh whole blood from healthy donors that had been already screened infectious marker. These whole bloods were removed plasma then added with additive solution. After fixed with the mixture of formaldehyde and glutaraldehyde, they were divided into three different values; normal, high and low value. The control products were measured 14 hematological parameters by using Sysmex XT 1800i automatic blood cell counter for 6 weeks for evaluated accuracy, precision and stability of control products by using percentage of coefficient variation (CV). The results of preparing 3 level value of hematologic control materials of WBC count, RBC count, Hb, Hct, MCV, MCH, MCHC, platelet count, RDW, % neutrophil, % lymphocyte, % monocyte, % eosinophil and % basophil by using fresh whole blood donors illustrated that the percentages CV of high value control material of hematologic parameters were 8.82, 1.06, 1.22, 4.23, 4.8, 0.76, 4.76, 6.28, 4.89, 5.07, 10.11, 27.1 and 13.91, respectively. The percentages CV of normal value control material were 4.73, 0.85, 1.29, 4.05, 3.53, 0.84, 3.82, 9.84, 4.37, 5.20, 8.23, 10.3 and 6.33, respectively. Including the percentages CV of low value control material were 3.0, 1.47, 3.58, 4.33, 3.86, 2.43, 4.62, 7.75, 2.88, 3.11, 5.69, 9.7 and 11.37, respectively. These results indicated that the percentages CV was less than the accepted value of Sysmex XT 1800i that WBC count and platelet count were less than 10, RBC count, Hb, Hct, MCV, MCH, MCHC and RDW were less than 5, while % neutrophil, % lymphocyte, % eosinophil were less than 15 and % monocyte was less than 40. This study showed that in-house hematological control products was suitable for internal quality control material at least for 5 weeks without any significant change from the original ( P > 0.05), except WBC count, Hct, % monocyte in the high value control, Hct, MCV, MCHC, % monocyte in normal value control and Hct, MCV, MCHC, RDW, % eosinophil in low value control.
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