Anti-Proliferative Mechanism of BmKn2 Peptide on Colon Cancer Cells

Pornpimol Treeyos, Teerakul Arpornsuwan, Suthathip Kittisenachai, Sittiruk Roytrakul

Abstract


Colon cancer is one of the most fatal cancers in the world. A scorpion venom peptide BmKn-2 showed potent cytotoxic effects towards human cancer cells with the concurrent induction of apoptosis. The aim of this study was to investigate the protein expression profiles associated with anti-proliferative effects of synthetic BmKn2 peptide in human colon cancer SW620 cells using shotgun proteomics technique. MTT assay demonstrated cytotoxic activity of BmKn2 with IC 50 of 40 µM in SW620 cells at 24h. SDS-PAGE analysis showed at least 4 higher intensity protein bands in BmKn2-treated than in untreated cells. LC-MS analysis of these protein bands in both samples identified 31 proteins which are differentially expressed. These proteins have been implicated in various cellular processes including transcription, metabolic process, cell proliferation, transport, cytoskeleton, immune response and cell signaling. Analysis based on the STITCH 4.0 database predicted the interaction of SUPT16H (SUPT16H), HEAT repeat-containing protein 6 (HEATR6), Nucleosome assembly protein 1-like (NAP1L3), Wilms tumor 1 (WT1) and synaptophysin, isoform CRA_a (SYP) with the tumor suppressor P53 (TP53). Taken together, BmKn-2 peptide exerts selective cytotoxic effects on human colon cancer cells by inducing P53 tumor suppressor pathway. It shows great promise as new potential therapeutic agent for colon cancer, with minimal effects on human healthy tissue and provides a framework for the development of peptide-based anticancer technologies.

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