Effect of Anticoagulants and Pre-analytical Time on Serum and Plasma Growth Factor Analysis

Mewadee Preecha, Tulyapruek Tawonsawatruk, Apirom Vongsakulyanon


Growth factors in blood are important for wound healing and contributed mainly from platelets. Currently, there is no consensus about measurement method and suitable anticoagulant for growth factor analysis. One hundred blood samples (ten collections) were drawn into various blood collecting tubes including clot activator, plain, citrate, heparin, and ethylenediaminetet­raacetic acid (EDTA). Serum or plasma obtained at 15 minutes, 4, 8 and 24 hours were analyzed for the presence of growth factors. Three platelet growth factors, [platelet-derived growth factor (PDGF)-AA, PDGF-BB and epidermal growth factor (EGF)], and erythropoietin were increased in the concentration with time in serum but unchanged in plasma. Other growth factors showed no difference between serum and plasma. Furthermore, serum PDGF-AA, PDGF-BB, and EGF showed moderate correlation with platelet count. In conclusion, types of anticoagulant, time before analysis and platelet count affected the concentration of some growth factors in blood.

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