Evaluation of Low Concentration Normal Saline Technique for Microfilaria Detection in Migrant Workers at PakTho Hospital, Ratchaburi Province

Kanpana Taokaew, Duangnate Pipatsatitpong

Abstract


Elephantiasis in Thailand is caused by filarial nematodes; Wuchereria bancrofti and Brugia malayi which are spreading world-wide including among migrant workers in Thailand. This study aimed to evaluate the use of the newly developed low concentration normal saline technique (LNSS technique) for detection of microfilaria in migrant workers at PakTho Hospital, Ratchaburi Province. A total of 1,967 blood samples collected from migrant workers at PakTho Hospital in 2016 were screened for microfilaria by thick smear, buffy coat capillary tube and LNSS technique. The results showed that 56 (2.8%) positive microfilaria cases were detected by LNSS technique but not by the thick smear and the buffy coat capillary tube methods. Comparison between LNSS technique and microfilaria confirmatory test showed that the sensitivity, specificity, positive predictive value, negative predictive value and accuracy were 100%, 100%, 100%, 100% and 100%, respectively. In conclusion, LNSS technique is easy, inexpensive and efficient for the microfilaria screening test. This technique should provide a suitable alternative method that can be applied to screen microfilaria in immigrant workers.


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