Neutrophil Chemotactic Distance

Natnicha Paungpan, Manatsaphon Sukmak, Nateelak Kooltheat, Thitiya Luetragoon, Kanchana Usuwanthim

Abstract


Neutrophils are  the  most abundant of  granulocytes in  blood  circulation. They  can migrate  through the  blood  vessels directed  to site  of  infection  by  chemoattractant inducing neutrophil chemotaxis, and  then  play  an  important role  in  microbial phagocytosis. Since neutrophil chemotaxis  is important in the innate  immune  response,  the  defect  of chemotaxis can  reduce  ability  in  phagocytosis  and  cause  severe  microbial  infection.  Here  we  study  the chemotaxis of  neutrophils. The  purpose   of  this  study   was  to  determine the  average  value of neutrophil chemotactic distance, Neutrophils were isolated from peripheral blood of 20 healthy student volunteers  from  Medical  Technology, Faculty  of  Allied  Health  Sciences,  Naresuan University. All  were  20-25  years  old  and  without   inflammation and  infection, Cells  were isolated  by density  gradient  centrifugation using  PolymorphprepTM,  The  purified  neutrophils were  then  stimulated  by  N-formylmethionine-leucyl-phenylalanine  (fMLP), an  end-target chemoattractant, to  observe   neutrophil chemotaxis   by  under  agarose  assay  using  dimethyl sulfoxide  (DMSO) as a negative control. The result showed that the average  value of neutrophil chemotactic distance  is 1,134   p..tm  (95%   CI;  1,134-1,414). This  value  might  be  used  as neutrophil chemotaxis  data  which  can  be applied  for  further study  on  neutrophil chemotaxis defect   patients  such  as  those  with  leukocyte adhesion deficiency  (LAD),  Chediak-Higashi syndrome and  hyper-lgE (Job) syndrome.


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