Real-Time Polymerase Chain Reaction assay for detection of Hepatitis B Virus DNA

Kasama Supanaranond, Waraphorn Fukpho, Mudtika Fungkrajai


This study was carried out in 200 blood donors at Thammasat Chalermprakiat Hospital. The ARCHITECT® HBsAg with the principle of Chemiluminescence Microparticle Immunoassay (CMIA) was used for HBsAg detection in blood donor screening. The DNA was extracted from plasma and determined by Real-Time Polymerase Chain Reaction method. The primers and TaqMan probe were derived from S gene. The HBsAg was detected in 3 blood donor samples (1.5%). The HBV DNA was detected in 5 blood donor samples (2.5%) with range 13-5687541 copies/μl. All 3 samples with detectable HBsAg were also detectable HBV DNA. Among 197 HBsAg negative blood donors, the HBV DNA was detected in 2 samples (1.02%). The data presented in this study indicate that the detection of HBsAg alone is not sufficient for blood safety. The introduction of HBV DNA as an additional marker for improve blood safety in transfusion is suggested.

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