Rapid Haemocultures Reported

Sujitra Manakul, Natthiya Inthachang

Abstract


Septicemia or sepsis refers to the presence of pathogenic organisms in the bloodstream with related clinical symptoms causing death. Rapid and accurate identification of a causative agent including antibiogram is, therefore, important. The objective of this study was to compare the results of bacterial identification and antimicrobial susceptibility testing (AST) obtained from the standard (18-24 hours incubation) and the modified (8 - 10 hours incubation) methods. The experiment was performed with 90 bacterial isolates acquired from 89 hemoculture positive samples from Surath-thani Hospital during April 2007 to June 2008.The results showed that 66 isolates (73.3%) were gram negative and 24 isolates (26.7%) were gram positive bacteria. Identification of these bacteria by the standard and modified methods yielded the same results for 64 isolates (97%) in gram negative and 23 isolates (95.8%) in gram positive bacteria. McNemar statistical test revealed no significant difference (p ≥ 0.05) between the two methods in term of identification, although the standard identification of one sample revealed two bacterial species, Escherichia coli and Klebseilla pneumoniae, while the modified method identified as Enterobacter aerogenes. Interestingly, AST of the standard and modified methods gave identical results for 66 isolates (100%) in gram negative and 18 isolates (75%) in gram positive bacteria. Statistical analysis of the AST showed significant difference between the two methods (p ≤ 0.05). Our results suggest that the modified method can be applied in laboratory to reduce the turnaround time for identification of both gram positive and gram negative bacteria. However, this modified method is not suitable for mixed culture specimen. For the AST, the modified method can be used only in gram negative bacteria.


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