Biological Safety Cabinet : The Using and Performance Verification

Boonchuay Eampokalap

Abstract


Biological Safety Cabinet (BSC) is an important containment for working safely with infectious microorganisms. Various types of BSCs provide varying degrees of personnel, environmental, and product protection. The duct connection to the outside environment for class II type A1 or type A2 BSC is through the use of thimble. BSCs should be situated remote from potentially disturbing air currents. During working, appropriate personal protective equipments should be worn. Arms should be moved in and out slowly. In case of spillage inside, the cabinet should continue to operate and clean up with disinfectant should be performed. The BSC should be verified (physical testing) compliance to the international standards at the time of installation, after has been relocated or repaired and at least annually by the competency engineer. The physical testing specified by NSF/ANSI Standard 49 includes inflow velocity, downflow velocity, exhaust velocity, smoke test and HEPA filter leak test. The test report should include cabinet information, standard used, test parameter, test method, test result, acceptable range, and conclusion (pass / fail).


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