Comparison of Non-Barbital and Barbital Buffer in the Determination of Alpha- -Macroglobulin by Agarose Gel Rocket mmunoelectrophoresis for the Replacement of Barbital Buffer

Yaovamarn Chantaranipapong, Somchai Pooduang, Talabporn Harnroongroj, Rungsunn Tungtrongchitr, Chaowanee Chupeerach

Abstract


The measurement of serum alpha-2-macroglobulin protein by the rocket immune-electrophoresis (R EP) assay employed an electro-immuno diffusion method. Barbital compound buffer (5, 5-diethylbarbituric acid, barbitone and sodium barbital) or veronal buffer was used as a standard diluent buffer for RIEP. These substances are designed as controlled substances with their drug abuse properties and toxicity in laboratory which brought to difficult import. To compare the alternative buffer and barbital buffer used for RIEP, the researcher then conducted the determination of serum protein alpha macroglobulin levels in 4 types of buffer, namely barbital buffer, Tris-barbital buffer, Tris-tricine, and Tris-azide buffer. In the running of rocket immunoelectrophoresis with 1.5% agarose and various voltage, Tris-tricine reveals the comparable ability to the standard barbital buffer.

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